1445 Expression Change of Adenosine Receptors in Human Chronic Periodontitis

Saturday, March 6, 2010: 11:45 a.m. - 1 p.m.
Location: Exhibit Hall D (Walter E. Washington Convention Center)
Presentation Type: Poster Session
C.-X. SUN, N.R. WALL, N. ANGELOV, C. RIRIE, J.-W. CHEN, D.S. BOSKOVIC, and J.M. HENKIN, Loma Linda University, Loma Linda, CA
Objective: Chronic periodontitis, affecting 5-15% of the adults in the U.S., is a chronic disease characterized by inflammatory cell accumulation in the extravascular gingival connective tissue. The molecular mechanisms for this dis-order are not well understood, and gingival recession occurs usually in spite of treatment. To elucidate the etiology of periodontitis, this study focused on the adenosine receptor (ADOAR) expression profiles (ADOAR1, ADOAR2A, ADOAR2B and ADOAR3) in periodontal diseased tissues. Methods: We measured adenosine receptor gene expression levels in hu-man gingiva from 15 patients with healthy gingival tissues (control group) and 15 patients who exhibited severe chronic periodontitis (test group), using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Results: The cycle numbers to reach crosspoint for ADOAR1, ADOAR2A, ADOAR2B and ADOAR3 are 25.36, 26.58, 20.96 and 21.27, respectively, in healthy gingiva. The expression pattern changed in human chronic periodontitis, the ADOAR1 decreased 20%, ADOAR2A increased 2.5-fold, ADOAR2B increased 3.7-fold, and ADOAR3 decreased 70%. Conclusions: Inflammation of the gingival tissue is associated with a) an unchanged expression of ADOAR1, b) an increased expression of ADOAR2A and ADOAR2B, and c) a decreased expression of ADOAR3. Logistic regression analy-sis indicated that the change in the expression patterns can be used to diagnose/predict periodontitis. This finding indicates that the adenosine receptor expression profile is changed in periodontitis with the potential for future clinical ap-plication.

Keywords: Gene expression, Inflammatory mediators, Periodontal disease and adenosine receptor
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