|Bioavailability of Xylitol-Containing Products in Saliva|
P. MILGROM, C. RIEDY, K.A. LY, M. ROTHEN, G. MUELLER, M. HAGSTROM, L. ZHOU, E. TOLENTINO, and M.C. ROBERTS, University of Washington, Seattle, USA|
Objectives: This study examined the presence and time course of xylitol concentrations in saliva delivered via a variety of xylitol-containing products.
Methods: Adults (N=15), using a within-subjects design, received three xylitol-containing products [xylitol pellet gum (2.6 g xylitol), xylitol gummy bears (2.6 g xylitol), and commercially available xylitol stick gum (3.0 g xylitol)]. Subjects consumed one xylitol product per visit with a 7-day interval between each product. A standardized protocol was followed for each product visit. Product order was randomly determined at the initial visit. Saliva samples (0.5 to 1.0 ml) were collected at baseline and 10-12 time points after product consumption initiated (xylitol gums – 1, 2, 3, 4, 5, 7, 9, 11, 13, and 15 minutes; xylitol gummy bears – after 1st gummy bear, after 2nd gummy bear, 2, 4, 6, 8, 10, 12, 14, and 16 minutes). Concentration of xylitol in treated saliva samples was analyzed using isocratic high-performance liquid chromatography (HPLC) separation of sugars. Area under the curve (AUC) for determining the average xylitol salivary concentration over the total sampling period was calculated for each product.
Results: All xylitol products (pellet and stick gums, gummy bears) had similar time curves with two xylitol concentration peaks during the sampling period. The mean first peak was at 4 minutes [pellet gum (6.8 ng/ml); gummy bears (7.2 ng/ml); and stick gum (6.0 ng/ml)]. The mean second peak was at 13 minutes [pellet gum (4.2 ng/ml); stick gum (3.4 ng/ml)], and at 14 minutes [gummy bears (4.0 ng/ml)]. Total AUC for the products did not differ significantly (pellet gum – 34.72, gummy bears – 39.74, and stick gum – 30.34).
Conclusion: Despite differing modes of delivery, these xylitol-containing products had similar time curves and mean xylitol concentration peaks suggesting that their effects (reduction in bacteria levels or decay) should also be similar.
NIDCR-NIH U54 DE14254
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