|2391 Immunodetection of EGFR in Squamous Cell Carcinoma in Mexican Patients|
C. TORRES CASTRO, C. C. ESQUIVEL CHIRINO, and E. LEYVA HUERTA, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico|
Objectives: Epidermal Growth Factor Receptor (EGFR), a 170-180kD transmembranal glycoprotein, is commonly expressed highly in the cell membrane of all squamous cells carcinomas (OSCCs) and it has been implicated in the control of cell proliferation, disease progression and poor survival, metastasis and angiogenesis. Itxs expression is associated with a poor prognosis in some tumors. The main goal of this study was to determine inmunohistochemically the expression of EGFR and to observe it there is a relationship between the expression and the differentiation in oral squamous carcinoma of Mexican patients. Methods: Formalin-fixed and paraffin-embedded pathology specimens blocks from 56 cases with OSCCs (1994-2005), from Department of Oral Pathology at School of Dentistry were used in this study. 5 samples of normal oral mucosa specimens were used as control. For each case hematoxylin and eosin staining for one tissue section was performed for histological review . Their antigens were retrieved with proteinase K, and the inhibition of the endogenous peroxidase with 3% hydrogen peroxide. Immunohistochemical staining was obtained using a monoclonal antibody against EGFR overnight at 4°C. Biotin-labelled horse anti-mouse immunoglobulin G antibody was applied for 1 h, followed by avidin-biotin peroxidase complexes for 30min. Diaminobenzidine was used as the final chromogen, and hematoxylin was used to counterstain. Clinical data were derived from retrospective chart reviews patient age, sex, primary tumor site. Results:50% of cases were well differentiated 39.28% moderately differentiated and 10.71% poorly differentiated. EGFR was expressed highly, a positive reaction was found in the majority of (OSCCs) in cytoplasm and cell membrane of cancer cells. Overexpression of EGFR is correlated with poor tumor differentiation. Conclusions: These results indicate that the overexpression of EGFR in tumors was much greater than that in normal tissue samples. Overexpression of EGFR is correlated with poor tumor differentiation.
|Seq #240 - Premalignant Oral Lesions and Oral Cancer|
3:30 PM-4:45 PM, Friday, March 23, 2007 Ernest N. Morial Convention Center Exhibit Hall I2-J