|0581 The Effects of Strontium Citrate on Osteoblast Proliferation and Differentiation|
A. BEUTTENMULLER, and R. DZIAK, SDM College of Dental Sciences, Buffalo, NY, USA|
Recent research has shown that stable strontium as well as some strontium salts increase bone metabolism in osteoblasts obtained from rodent calvaria cultures and that this element, particularly in the form of strontium ranelate, may be useful in the treatment of osteoporosis and other diseases of bone remodeling. Objectives: To assess the effects of strontium citrate, a product commonly available as a nutritional supplement, on the proliferation and differentiation of osteoblastic cells obtained from human alveolar bone. Methods: Primary osteoblasts, obtained with patients' consent from human alveolar bone residues at the time of third molar extractions and explanted in culture, were seeded at a concentration of 105 cells/ml in 24 well polystyrene plates in BGJb media containing 10% FCS. After the cells reached a semiconfluent stage, strontium citrate (bibasic anhydrous, pure grade from Jost Chemical) at various concentrations (0.05-1.0 mM) was added. The experiments performed included a MTT test which assesses viability/proliferation with a measure of mitochondrial activity and a biochemical assay for alkaline phosphate activity, an early maker of osteoblastic cell differentiation. Measurements were performed at 24, 48, and 72hrs. Results: The data were analyzed using ANOVA. The MTT test showed the cells to be responsive to strontium citrate with significant increases (p<0.05) in cellular activity/proliferation at 24 and 48 hrs. Alkaline phosphatase activity was significantly enhanced (p<0.05) at 48 and 72 hrs with strontium citrate concentrations in the test range. Conclusion: The data support the hypothesis that strontium citrate increases the proliferative/alkaline phosphatase activity of human osteoblastic cells from alveolar bone. The results validate previous research that has been done with other forms of strontium in clinical studies and rodent calvarial cells and indicates that strontium citrate could be a promising agent in treating oral as well as systemic bone disorders.
Funded by Dental Medicine Centennial Fund
|Seq #87 - Cell differentiation|
2:00 PM-3:15 PM, Thursday, March 22, 2007 Ernest N. Morial Convention Center Exhibit Hall I2-J