1162 Effect of xylitol on a model of oral biofilm
M.-C. BADET, U.F.R. of Odontology, Bordeaux Cedex, France

Objectives: The aim of the present study was to examine whether xylitol, at different concentrations, inhibits the formation of an experimental model of oral biofilm. Materials and methods: Biofilms of six bacterial species (Streptococcus mutans, Streptococcus sobrinus, Lactobacillus rhamnosus, Actinomyces viscosus, Porphyromonas gingivalis, Fusobacterium nucleatum) were prepared on hydrodyapatite (HA) discs following the Zürich model. Xylitol was tested at a concentration of 1% and 3%. At the end of their designated incubation times, some HA disc were destined for laser scanning microscopy (CLSM) and other were scraped with a sterile surgical instrument. Aliquots of harvested biofilm were diluted and plated onto specific media. After a 48 hrs anaerobic incubation at 37°C, colony-forming units were counted. Results: CLSM showed that only poor quantity of isolated bacteria was observed on the surface of hydroxyapatite discs. Culture of harvested biofilm showed an inhibition of the growth of the different species included in the biofilm. Conclusions: There is a clear inhibiting effect of xylitol on the formation of the experimental biofilm. This study shows that xylitol is not only efficient on the acid production of cariogenic bacteria, but also on the formation of a multispecies biofilm; it confirms the relevance of the use of this polyol for the prevention of oral diseases caused by dental plaque.

Seq #126 - Microbes and Oral Infections
3:30 PM-4:45 PM, Thursday, March 22, 2007 Ernest N. Morial Convention Center Exhibit Hall I2-J
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