0501 Amorphous Calcium Phosphate-Casein Phosphopeptide Paste: Effect on Enamel Caries Formation
J. HICKS, Baylor College of Medicine, Houston, TX, USA, and C. FLAITZ, University of Texas Dental Branch - Houston, USA

OBJECTIVE: This in vitro study evaluated the effect of a casein phosphopeptide-amorphous calcium phosphate paste (CPP-ACP) on human enamel caries formation using polarized light microscopy. METHODS: 12 human teeth with sound enamel surfaces were sectioned into 4 portions. Each portion from a single tooth was assigned to a treatment group: 1) CPP-ACP (MI Paste, GC America, n=12); 2) 0.05% sodium fluoride rinse (NaF, ACT McNeil-PPC, n=12); 3) CPP-ACP and NaF; 4) No Treatment Control (n=12). CPP-ACP and NaF tooth portions were treated with the appropriate assigned agent(s) for 60 seconds, followed by air-water rinsing for 60 seconds, and then exposed to synthetic saliva for 24 hours. CPP-ACP and NaF treatment, rinsing and synthetic saliva exposure were repeated on a daily basis over a 14-day period. No treatment control tooth portions were exposed to synthetic saliva only for a 14-day period. In vitro enamel caries were created over a 10-day period (alternating 16 hours demineralization followed by 8 hours remineralization). Longitudinal sections (3 sections/tooth segment) were obtained and lesion depths were determined using polarized light microscopy (n=36 lesions/group, ANOVA, DMR). RESULTS: Mean lesion depths were: 1) No Treatment Control Group: 262±22um; 2) NaF Rinse Group: 156±17um; 3) CPP-ACP Group: 106±11um; 4) CPP-ACP & NaF Group: 88um±12um. Mean lesion depth reductions compared with the No Treatment Control Group were: 40% for NaF Rinse (P<.05, ANOVA, DMR), 59% for CPP-ACP (P<.05, ANOVA, DMR), and 66% for CPP-ACP & NaF (P<.05, ANOVA, DMR). CONCLUSIONS: CPP-ACP paste alone and in combination with NaF markedly enhanced the resistance of enamel surfaces to in vitro caries formation. Bioavailable calcium and phosphate in CPP-ACP may be an important factor in reducing susceptibility to a cariogenic challenge.

Seq #71 - Fluorides/Other Caries Prevention
2:00 PM-3:00 PM, Thursday, 9 March 2006 Dolphin Hotel Pacific Hall

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