0108 Biocompatibility of MTA, Portland cement and modified Portland cement
K.P.S. FERNANDES, M.D. MARTINS, S.H. REDA, L. MOTTA, V.C.S. PAVESI, and S.K. BUSSADORI, UNINOVE, São Paulo, Brazil

Objective: evaluate the biocompatibility (tissue reaction in rats subcutaneous) and cytotoxic effects (in vitro) of MTA (Pro-Root), Portland cement and modified Portland cement (gypsum added-0,0599g/1,0001g). Methods: For in vivo analysis, polyethylene tubes (3 containing the materials and 1 empty - control) were implanted in subcutaneous of 28 rats. After 7, 14, 30 and 60 days the tubes and surrounding tissue were prepared for histological examination (3mm sections).The qualitative evaluation was done concerning the type and the intensity of the inflammatory infiltrate, fibrosis, material overwhelming and tissue necrosis. The cytotoxic effects were evaluated using NIH-3T3 cells. For the growth and viability curves, each substance (0,06g ) was applied directly on the top of glass cover slips into Petri dishes (60 mm) containing 10 4 cells (control cultures received only plain cover slips). After 1, 3, 5 and 7 days (long-term assay; survival), the cells were counted, in triplicate, using the Trypan blue dye exclusion assay. Results: The histological analysis showed moderate inflammatory response at the first 7 days in all groups (more severe on modified Portland cement). After 15 days the control group, MTA and Portland cement showed a mild inflammatory process while modified Portland group still showed moderate inflammatory process. After 30 and 60 days, all materials were similar. During the all experimental period there was no difference in cell survival between Portland cement and modified Portland cement groups (Kruskal-Wallis p<0.05). However, there was difference between modified Portland cement and MTA group (at day 5) and between Portland cement group and MTA group (at days 3 and 5). Control group showed significantly higher viability percentages than all the experimental groups after 3 days (Kruskal-Wallis p<0.05). Conclusion: According to these experimental conditions, the tested materials disclosed similar results in vitro cytotoxicity and in vivo biocompatibility.

Seq #12 - Endodontic Materials, Endodontic Instruments, Fluoride, Light-curing
1:00 PM-2:00 PM, Wednesday, 28 June 2006 Brisbane Convention & Exhibition Centre Exhibit Hall 1

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