0637 Nifedipine Does Not Alter Cyclins in NIFr or NIFn
R. TAKEUCHI, H. MATSUMOTO, and A. FUJII, Nihon University School of Dentistry at Matsudo, Chiba, Japan

Objectives: Our previous study demonstrated that human gingival fibroblasts originated from patients who developed gingival overgrowth by nifedipine (NIF) medication (NIF responder, NIFr), grew better in the presence of 1 mM NIF than those originated from patients non-reactive to NIF (NIF non-responder, NIFn). We have also reported that NIF activate PLD-dependent intracellular signaling in gingival fibroblasts. The present investigation was undertaken to clarify whether NIF affects to the factors related to cell cycle, such as cyclin A, B, D and E. Methods: Human gingival fibroblasts were obtained from gingival specimen and cells were grown in DMEM-10, supplemented with 10 % fetal calf serum, 100 mg/ml streptomycin, 100 U/ml penicillin G, and 0.2 mg/ml amphotericin B in Dolbecco's modified Eagle medium. Cell growth was measured in the presence of 1 mM and 10 mM NIF in DMEM-10 for 48 hours. After the RNA extraction, the expressions of cyclins A, B, D and E were studied using primer pair specific for cyclin A, B, D and E in RT-PCR with 33, 25, 21, and 30-cycle amplifications, and then analyzed on a 2 % agarose gel containing ethidium bromide. Results: 1 mM NIF treatment for 48 hours gave significant cell growth in NIFr, but not in NIFn. 10 mM NIF did not alter the growth in both NIFr and NIFn. 1 mM and 10 mM NIF treatments did not alter the expressions of cyclin A, B, D and E. Conclusions: These results indicate that the increased cell growth by 1 mM NIF is not due to the direct effect to the factors for cell cycle. This study was supported in part by a grant from the Japan Society for the Promotion of Science 11671885 (H.M.) and 13671983 (A.F.).

Seq #71 - Oral Tissues, Pharmacology
11:00 AM-12:15 PM, Thursday, 26 June 2003 Svenska Massan Exhibition Hall B

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