|0636 Baicalin Inhibits the Expression of PGE2 and MMP-3 in Human Periodontal Ligament Cells|
C.Z. LI1, Z.G. CAO1, M. LI1, S.H. SHANG1, M.W. FAN1, E.F. CORBET2, and L.J. JIN2, 1 Wuhan University, China, 2 University of Hong Kong, Hong Kong|
Baicalin is an extract from the dry roots of Scutellaria baicalensis (Huangqin, a traditionally used Chinese medicinal herb). Objective: To evaluate the biologic effect of baicalin on the expression of PGE2 and MMP-3 by cultured periodontal ligament cells (PDL cells) stimulated with IL-1b. Methods: Human PDL cells were obtained from the periodontal membrane of first premolars extracted for orthodontic purpose and they were cultured with DMEM following routine protocol. PGE2 level in cell culture fluid was measured by ELISA. MMP-3 expression was measured using immunohistochemistry and quantitative image analysis system. Monensin was used as a positive control for inhibition of MMP-3 release by the PDL cells. Results: PGE2 level in culture fluid significantly increased from 15.00±0.82 pg/ml at baseline to 84.50±2.38 pg/ml (p<0.01) when the PDL cells were stimulated with 1.0 ng/ml of IL-1b for 24 h. When the cells were stimulated with IL-1b (1.0 ng/ml) and treated with baicalin (0.1 µg/ml) for 24 h, PGE2 level decreased significantly from 84.50±2.38 to 32.40±4.38 pg/ml (p<0.01). At baseline, no expression of MMP-3 was observed in PDL cells, while positive expression was found when the cells were stimulated with IL-1b in a dose-dependent manner. When the cells were stimulated with IL-1b (1.0 ng/ml) for 12 h and subsequently treated with baicalin (0.1 µg/ml) or monensin 5.0 µM for 12 h, consistent expression of MMP-3 was observed at 12 h and 24 h. In contrast, MMP-3 expression was detected in those cells stimulated with IL-1b (1.0 ng/ml) for 12 h, while no expression was observed when these cells were stimulated for an additional 12 h. Conclusion: Baicalin may have an inhibitory effect on the expression of PGE2 and release of MMP-3 from human periodontal ligament cells. Supported by Science & Technology Grants, Hubei, China.
|Seq #71 - Oral Tissues, Pharmacology|
11:00 AM-12:15 PM, Thursday, 26 June 2003 Svenska Massan Exhibition Hall B