|4109 Oxidative Modification of Salivary Biomolecules with Therapeutic Levels of Ozone|
A. CLAXSON, C. SMITH, M. TURNER, C. SILWOOD, E. LYNCH, and M. GROOTVELD, Queens University, Belfast, United Kingdom|
Objectives: Previous studies have demonstrated that ozone (O3) exerts a powerful bactericidal action towards S. mutans, together with further micro-organisms associated with primary root carious lesions. Therefore, the application of O3 in dental practices may serve as a viable and convenient means of treating dental caries. In this study we have employed high field proton (1H) NMR spectroscopy to evaluate the oxidising actions of O3 towards salivary biomolecules.
Methods: Unstimulated human saliva specimens were collected from 12 patients and each of them was divided into two equivalent volumes (0.60 ml). The first of these was treated with O3  for a period of 10s, and the second group served as controls. Samples were then subjected to 1H NMR analysis at an operating frequency of 600.13 MHz.
Results: In addition to the complete oxidative consumption of salivary pyruvate (mean ± s.e. salivary level prior to treatment 1.75 ± 0.62 mM) and partial oxidation of methionine, results acquired revealed (1) marked elevations in the concentration of formate (a product derived from the oxidation of carbohydrates), (2) substantial reductions in lactate level (up to 92%) via its oxidation to pyruvate, which in turn is oxidised to acetate and CO2, (3) the oxidation of 3-D-hydroxybutyrate to acetoacetate, which liberates acetone on decomposition, (4) the generation of allantoin from salivary urate (up to levels of 40 mM), (5) the production of low-molecular-mass saccharide fragments from hyaluronate, and (6) oxidation of malodorous trimethylamine (initial salivary concentration 78 ± 23 mM) to its corresponding, non-malodorous N-oxide.
Conclusions: 1H NMR analysis provides a wealth of information regarding the nature and extent of salivary biomolecule oxidation by O3 which are of much relevance to its potential therapeutic actions in the oral environment.
 HealOzone Unit, CurOzone, U.S.A.
|Seq #385 - Salivary Composition/Physiology/Pathology|
3:45 PM-5:45 PM, Saturday, 9 March 2002 San Diego Convention Center Room 1A (Upper Level)