Objectives: To compare the antimicrobial effect of BioPure MTAD (Dentsply Tulsa Dental, USA), SmearClear (SybronEndo, USA), 2.5% NaOCl (Caglayan Kimya, Turkey), 2% chlorhexidine gluconate (Drogsan, Turkey) and 10% boric acid (Merck, Germany) solutions on Enterococcus faecalis using an in vitro modified tooth model.

Methods: Forty-eight human root segments (7-mm height) were prepared and their pulpal lumens were standardized to a 1.3-mm diameter with using ISO size 130 Peeso Reamer (Mani, Japan). After removing smear layer with sequentially using 17% EDTA and 5.25% NaOCl for 5 min in an ultrasonic bath, all segments were sterilized (121°C, 20 min). Each segment was mounted in well of 24-well tissue culture plates using wax and received 15µl of overnight cultures of E. faecalis that is optically standardized with using a spectrophotometer (OD₆₀₀= 500) ever day for 14 consecutive days and kept at 37°C. Each lumen treated with two successive 5-minute flushes with the experimental irrigants or saline. Dentin chips were obtained from inner canal walls using Peeso Reamers (Size 150 and 170) and were transferred to vials containing phosphate-buffered saline (PBS) and glass beads, and vortexed. Serially diluted and 25 microL droplets were seeded on TSA plates. The plates were incubated (37° C, 24h) and colony-forming units were counted. Data were analyzed using Kruskal-Wallis and Mann-Whitney U tests (alpha = 0.05).

Results: SmearClear and chlorhexidine solutions demonstrated significantly more antimicrobial activity than the BioPure MTAD, 2.5% NaOCl, 10% boric acid and saline control. Boric acid solution showed the least antibacterial activity and performed similarly to the saline control.

Conclusions: Surfactant enhanced 17% EDTA solution, SmearClear and chlorhexidine solutions are more effective disinfectants on E. faecalis.

Acknowledgements: Authors would like to thank to the Dentsply Tulsa Dental, SybronEndo, Caglayan Kimya and Drogsan companies for their material support.

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