In view of the Minimal Invasive Dentistry approach specific differentiation between infected and affected dentin would significantly contribute to prevent over-excavation of caries lesions. Available caries detector solutions are subject to discussions because of over-staining of dentin in caries lesions. Objectives: of the study was to evaluate the performance of the new caries indicator BV-1 (3M ESPE) to discriminate between infected dentin in excavated caries lesions. Methods: Caries lesions in freshly extracted human teeth were excavated by dentists with either a slow drilling device or with a hand excavator. BV-1 was used to detect remained infected dentin. 60 Biopsies were taken from stained and unstained sites with bur No.5. Dentin samples were transferred to 1 ml of brain-heart bullion and in duplicates the total anaerobe counts were determined on brain-heart agar. A two sample t-test (p<0.05) was used to compare the bacterial counts found in biopsies taken from stained and unstained sites, respectively. Sensitivity and specificity of BV-1 was calculated by using 100CFU/biopsy as a threshold concentration. Results: In discoloured sites of excavated caries lesions significantly more total anaerobe bacteria have been found (p<0.05). A sensitivity of 83% and 84% and a specificity of 68% and 71 % was observed with BV-1 to detect infected dentin (>100 CFU) after bur or hand excavator treatment, respectively. Conclusion: BV-1 showed in this in vitro study a promising performance to detect infected dentin in excavated caries lesion. Further clinical studies are needed to explore the diagnostic performance of BV-1 in vivo.

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