| 0927 Modulation of Mesenchymal Stem-Cell Growth-Factors by Emdogain® and EMD Fractions | ||
|
T. RAKMANEE1, N. DONOS1, M. DARD2, and I. OLSEN1, 1Eastman Dental Institute for Oral Health Care Sciences, London, United Kingdom, 2Institute Straumann AG, Basel, Switzerland OBJECTIVES: Emdogain® (EMD) is a heterogeneous mixture of enamel matrix proteins used clinically to promote periodontal regeneration in vivo. EMD stimulates cell proliferation, differentiation and secretion of growth factors (GF) in vitro. Mesenchymal stem cells (MSC) are likely to be the source of new periodontal tissue, but the effects of EMD and of partially-purified EMD fractions on these cells are not yet known. Since GF have a major role in tissue regeneration, the objective of this study was to measure the effects of EMD on GF secretion profiles of MSC using a flow cytometry based Multiplex Bead Assay (MBA). METHODS: MSC were cultured in the absence (control) and presence of EMD, recombinant human (rh) amelogenin, and EMD fraction-A (20-25kDa) and fraction-C (average 5kDa) for 6, 24, 48 and 72h. Culture supernatants were used to simultaneously measure eight GF: platelet-derived growth-factor-AB(PDGF-AB); keratinocyte growth-factor(KGF); osteoprotegerin(OPG); fibroblast growth-factor-2(FGF-2); bone morphogenetic protein-2(BMP-2); tissue inhibitor of metalloprotease-1(TIMP-1); angiopoietin-1(Ang-1); vascular-endothelial growth-factor(VEGF). Tyramide Signal Amplification was used in conjunction with the MBA to increase assay sensitivity. RESULTS: EMD progressively up-regulated VEGF and OPG secretion compared with rh-amelogenin, fraction-A and fraction-C. However, fraction-A and fraction-C stimulated the secretion of FGF-2 and KGF, while incubation with rh-amelogenin increased OPG, BMP-2 and Ang-1 secretion by MSC. CONCLUSION: This is the first report to demonstrate that EMD induces the secretion of multiple GF by MSC, although each GF was affected to a different degree, with VEGF and OPG being particularly significant and indicating the pivotal role of angiogenesis and osteogenesis in bone healing. The partially purified EMD fractions also selectively modulated GF secretion although each had a unique effect on the different GF profiles, suggesting that specific individual components of EMD might target distinct processes in regeneration of the soft and hard tissues of the periodontium. | ||
| Seq #98 - Periodontal Research: Tissue Culture, Regeneration 2:00 PM-4:00 PM, Friday, September 12, 2008 Queen Elizabeth II Conference Centre Theatre J | ||
|
Back to the PEF IADR 2008 Program
| ||