0771 The Effect of Amine Fluoride on Initial Biofilm Formation
E.-M. DECKER, G. MAIER, A. ELAYOUTI, M. BRECX, and C. VON OHLE, University of Tübingen, Tuebingen, Germany

 

Objectives:

Amine fluoride is a surface-active fluoride beneficial for dental plaque control and contained in a number of complex formulations of oral hygiene products.

The aim of the study was to investigate the effect of pure amine fluoride (Olaflur) on initial biofilm formation of the early human tooth colonizer, Streptococcus sanguinis, in comparison to the antibacterial gold standard chlorhexidine (CHX), an antiadhesive chitosan derivative (Chit) and to saline (NaCl) as negative control.

 

Methods:

In a preclinical biofilm model Strep. sanguinis cells were exposed to 0.2% Olaflur, 0.1% CHX, 0.2% Chit and NaCl (0.9%) for 2min. After suspension in sterile human saliva the streptococci were allowed to attach to human enamel and glass slides for 60min. These initial biofilms were characterized by monitoring the microbial vitality (V) after fluorescent staining (Syto9/propidiumiodide) as well as by bacterial cell counts (BC) on the substrata. Furthermore, the treated bacteria in the salivary suspension were enumerated (CFU) following to cultivation on agar plates. Means (n = 10) and corresponding 95% confidence intervals were calculated for each substrate / test substance combination.

 

Results:

           

 

V (%)

on the substrata

log BC

on the substrata

log CFU of the salivary suspension

enamel

glass

enamel

glass

Olaflur

2.6

(0.5-4.6)

1.3

(0.02-2.5)

1.9

(1.8-2.0)

1.4

(1.2-1.6)

0

(0-0)

CHX

14.3

(6.1-22.6)

23.6

(14.5-32.7)

2.6

(2.3-2.9)

2.5

(2.2-2.7)

7.1

(6.8-7.5)

Chit

71.3

(62.3-80.2)

61.1

(53.4-68.8)

2.5

(2.4-2.6)

2.1

(2.0-2.2)

8. 1

(7.9-8.3)

NaCl

70.2

(58.5-81.9)

67.7

(58.9-76.4)

3.5

(3.3-3.8)

3.7

(3.3-4.0)

8.1

(7.8-8.3)

 

Conclusion:

Compared to treatment of Strep. sanguinis cells with CHX, Chit, and NaCl, the exposure of the streptococci to the amine fluoride Olaflur resulted in a stronger antivital and antiadhesive effect on the initial biofilms on enamel and glass. Moreover, Olaflur demonstrated a maximum antibacterial effect under the experimental conditions.

Supported by GABA International, Switzerland.

Seq #83 - Microbiology - Disinfection, Antibacterial actions
11:30 AM-1:00 PM, Friday, September 12, 2008 Queen Elizabeth II Conference Centre Poster Hall 2

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