0768 Essential-oil and Delmopinol Mouthrinses: Effect on Streptococcus mutans and Lactobacillus
H. LUIS1, L.S. LUIS2, I. GASPAR2, M.G. SOARES2, and M. BERNARDO1, 1University of Lisbon School of Dentistry, Lisboa, Portugal, 2Instituto Piaget - Almada, Portugal

Objectives: An in-vitro study was developed to evaluate antimicrobial activity of an Essential-Oil mouthrinse - EO - (Cool Mint Listerine Antiseptic) and a Delmopinol mouthrinse - DM - (Decapinol) on Streptococcus mutans (SM) and Lactobacillus (LB) isolated from dental biofilm.

Methods: Samples were collected using a swab (BBLTM Culture Swab Plus, Becton Dickinson), from buccal surface of one quadrant of healthy adults with a minimum of 6 teeth per quadrant and periodontal pockets depth less than 5mm. After sample collection, SM and LB colonies were isolated and identified. Bactericidal effect was evaluated by disk diffusion assay for each of the mouthrinses under study, using a chlorhexidine mouthrinse – CHX - (Corsodyl Dental, GlaxoSmithKline) as a positive control and sterile distilled water as negative control. Since the studied mouthrinses have alcohol in its composition, a comparison between the mouthrinses and its correspondent alcohol content was performed, to estimate the additional inhibition obtained by the mouthrinses active ingredients other than the alcohol. Inhibition zones of bacteria around disks were measured in millimetres.

Results: Inhibition zones (in mm) for the mouthrinses and its alcohol content alone are presented in the next table. Water did not inhibit bacterial growth.

  EO EO-Alcohol (21.5%) DM DM-Alcohol (1.5%) CHX
SM 14.5 13.9 15.0 10.1* 14.8
LB 21.1 16.2* 21.0 11.5* 22.9

There are no statistically significant differences among mouthrinses for SM and LB inhibition (p>.05). Concerning mouthrinses and its alcohol content, statistical significance was found for the comparisons EO vs. EO-Alcohol and DM vs. DM-Alcohol marked, in the table, with an asterisk (*), (p<.05).

Conclusions: Study mouthrinses have shown in-vitro antimicrobial activity on isolated Streptococcus mutans and Lactobacillus. There were no statistically significant differences between EO, DM and CHX. The inhibition of EO and DM is superior to its alcohol content alone. (UICOB, FCT UNIT 4062)

Seq #83 - Microbiology - Disinfection, Antibacterial actions
11:30 AM-1:00 PM, Friday, September 12, 2008 Queen Elizabeth II Conference Centre Poster Hall 2

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