| 0342 Cytotoxicity of a silorane-based composite in a dentin barrier test | ||
|
G. SCHMALZ1, Z. ERGUCU2, and K.-A. HILLER1, 1 University of Regensburg, Germany, 2 Ege University, Izmir, Turkey Composite resin materials are applied onto the cut dentin and thus the risk for the dental pulp has to be assessed. Objective: To evaluate the cytotoxicity of a new low-shrinking composite resin based on silorane-technology together with appropriate self-etching dentin adhesives in comparison with a known composite resin in a dentin barrier test simulating the in vivo situation. Methods: Three-dimensional cultures of SV40 transfected bovine and human pulp derived cells were transferred into an in vitro dentin barrier test device with dentin slices of 200 and 500 m m thickness. After 24 h incubation at 37°C test materials (Hermes 134468, Hermes 151174; 3MEspe), the reference material (TerticCeram/Exite, Vivadent) as well as the positive (Vitrebond, 3MEspe) or the negative control (President, Coltene) were applied into the cavity part of the devices. The pulpal part of the device was perfused with cell culture medium (2 ml/h). After 24 h exposure, cell survival was measured by MTT tests. The negative control represents 100% cell vitality. At least 15 (bovine) and 5 (human) cell cultures per material were statistically analyzed (Mann-Whitney-test, a =0.05). Results: With 500 m m dentin slices the bovine cell survival rates for Hermes 134468 (97%) and for TetricCeram/Exite (99%) were not significantly different. Hermes 151174 reduced bovine cell activity to 83% (500 m m dentin) and 85% (200 m m dentin) but was not changed by TetricCeram 104% (200 m m dentin) compared to negative controls. Activity of human pulp cells on 500 m m dentin slices was 83% for Hermes 151174 and 99% for TetricCeram, being significantly different. Conclusions: The silorane based composite resin was not toxic in the formulation Hermes 134468 and slightly toxic in the formulation Hermes 151174. This difference is attributed to the different bonding agents used for Hermes 134468 and Hermes 151174. | ||
| Seq #33 - Dental Materials: Material/ Tooth Interface, Toxicity 4:00 PM-6:00 PM, Friday, 27 August 2004 Crowne Plaza Hotel SEDEF III | ||
|
Back to the Scientific Program Program
| ||