Objective: The aim of this study was to assess the pH and calcium ion release of three calcium hydroxide based root canal sealers—Sealapex (Kerr, USA), Acroseal (Septodont, France), and Apexit (Vivadent, Germany). Methods: After manipulation, the sealers were placed in 1-cm-long tubes measuring 4 mm in diameter and immersed in a glass flask containing 10 ml of bidistilled water, which was hermetically sealed and stored at 37 °C. The tubes were removed at the scheduled times (24h, 96h, 7, 15, and 28 days) and the water in which they were immersed was tested for pH with a pH meter and for released calcium by using a calcium measurement reagent and spectrophotometry. Statistical analysis was performed by ANOVA for each time interval studied, and individual comparisons were performed by using Tukey HSD Test. Results: Selapex produced an alkaline pH and released significantly higher calcium amounts compared with the other two sealers. Conclusions: On the basis of the results obtained, it was concluded that Sealapex presented the highest calcium and hydroxyl release. Apexit showed higher calcium release then Acroseal at the end of 28 days. Acroseal presented the least satisfactory results regarding the calcium and hydroxyl release.

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