OBJECTIVES: The aim of this study was to evaluate in-vitro decontamination degree of three implant surfaces colonized by P. gingivalis (P.G.) and treated using Erbium Yag Laser (De-Light, Sweden Marina, Italy). METHOD: The three trial groups were composed of twenty machined , twenty sandblasted and etched and twenty TPS. Every group was divided in two subgroups composed of ten implants: a sample and a control one. Bacterial strains (P. Gingivalis ATCC33277) were left in a 10 ml Schaedler broth solution, for a 48 hours incubation period with anaerobic conditions and 37°C temperature. Following, the implants were contaminated by P. gingivalis and washed three times with a 10 ml PBS sterile solution to eliminate non bound bacteria. The laser probe used was the FC90DE LIGHT, featuring an 80° bending radius with a twenty microseconds drive rate, and emission pulsated at ten Hz speed and 30 MJ power. The probe worked on implants with a 45° angle of incidence to the axial surfaces of the implant threads, developing apex-crown motions that lasted sixteen seconds. Evaluation of implant surfaces was made using a S.E.M. device , taking three size pictures (1000x, 6000x,11000x) and calculating the P.G. number on a 4800 nano meters surface utilizing a 10 m grid. Data were made statistically significant using Fisher test. A logarithmic conversion was made because of the asymmetrical distribution of bacterial counting. RESULTS: Counting results showed a 76.2% decontamination degree in machined implants trial group, a 90,9% decontamination degree in TPS implants trial group and 98,3% for sandblasted and etched implants trial group. CONCLUSION: These preliminary results may suggest the use of Erbium Yag laser as one of the standard peri-implant instrumentation to treat the implant surfaces affected by peri-implant diseases.

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