0331 Ultramorphological and Histochemical Changes Following Irradiation by Laser
E. ALTUNDASAR1, Z.C. CEHRELI1, B. ÖZÇELIK1, and K. MATSUMOTO2, 1 Hacettepe University, Ankara, Turkey, 2 Showa University, Tokyo, Japan

Objectives: The smear layer removal efficiency of Er:Cr:YSGG laser application and alterations caused on the root dentin was compared with the specimens prepared using Rc-Prep.Methods: 30 extracted single rooted human teeth with single canal were examined. 3 groups were formed to undergo different procedures for the aim of smear layer removal. Group1:5,25%NaOCl was used during preparation followed by a final irrigation with the same solution. Group2: 5,25%NaOCl and Rc-Prep were used in combination during preparation followed by a final irrigation of 5,25%NaOCl. Group3: 5,25%NaOCl was used during preparation followed by irradiation of laser and then a final irrigation of 5,25%NaOCl. Specimens were prepared for stereoscopy and SEM to be examined for the morphological changes and the absence of the smear layer whereas SEM-EDX method was utilised for the mineral analysis.Results: While Gr1 and Gr2 specimens that were only treated with chemical solutions exhibited heavy-homogeneous smear layer, there was relatively cleaner root surfaces in Gr3 but the total removal of the smear layer was not evident. The mineral analysis revealed that Gr2 and Gr3 specimens had higher mineral levels than Gr1 which was statistically significant. However no difference was detected between the Ca/P ratios of the 3 groups. Conclusions: Absence of a statistical difference among the mineral ratios of the three groups suggests that the change was not at molecular level.

Seq #32 - Craniofacial Biology, Mineralized Tissue, Pulp Biology
4:00 PM-6:00 PM, Friday, 27 August 2004 Crowne Plaza Hotel SEDEF I

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