| 0196 Therapeutic versus prophylactic omega-3 fatty acid in experimental periodontitis | ||
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S. VARDAR1, E. BUDUNELI1, O. TURKOGLU1, A.H. BERDELI2, H. BAYLAS1, and G. ATILLA1, 1 Ege University School of Dentistry Department of Periodontology, Izmir, Turkey, 2 Ege University School of Medicine Department of Pediatrics Molecular Medicine Laboratory, Izmir, Turkey Objectives: The aim of the present study was 1) to evaluate the possible effects of therapeutic usage of omega-3 fatty acid (O3) on the gingival tissue levels of prostaglandin E2(PGE2), prostaglandin F2α(PGF2α), leukotriene B4 (LTB4) and platelet activating factor (PAF) in endotoxin-induced periodontitis in rats, 2) to investigate whether prophylactic usage provides any additional benefits to therapeutic usage of O3. Methods: Experimental periodontitis was induced by repeated injection of E. coli endotoxin (LPS). Thirty-six rats were divided into 4 study groups as follows; saline, LPS, therapeutic O3 (TO3) and prophylactic plus therapeutic O3 (P+TO3) groups. In TO3 group, O3 was given to rats with experimental periodontitis for 15 days. In P+TO3 group, O3 was started 15 days before baseline, and then periodontitis was induced at baseline and O3 was continued for 15 days after baseline. On day 15 after baseline, all rats were sacrificed. PGE2, PGF2α, LTB4 and PAF levels in gingival tissue samples were analyzed by enzyme immunoassay and radioimmonoassay. Data were evaluated statistically by using parametric tests. Results: LPS injection resulted in significantly more bone loss than the saline controls (P<0.05). TO3 group exhibited significant decreases PGE2, PGF2α, LTB4, and PAF levels compared to the LPS group (P<0.05). PGE2 and PGF2α levels in TO3 group were similar to the saline group (P>0.05), while LTB4 and PAF levels were statistically higher than the saline group (P<0.05). Prophylactic plus therapeutic O3 provided similar levels of all these mediators to the saline controls (P>0.05). Conclusion: Therapeutic O3 significantly reduced the gingival tissue levels of PGE2, PGF2α, LTB4, and PAF in experimental periodontitis. Furthermore, prophylactic usage of O3 provided additional beneficial effects to the therapeutic administration by decreasing the gingival tissue levels of these mediators to levels of healthy tissue. | ||
| Seq #20 - Periodontal Research - Therapy 9:00 AM-11:00 AM, Friday, 27 August 2004 Crowne Plaza Hotel SEDIR I | ||
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