| 0481 Relationship between smoking during pregnancy and subgingival microbiota | ||
|
N. BUDUNELI1, H. BAYLAS1, E. BUDUNELI1, H.O. TURKOGLU1, and G. DAHLEN2, 1 Ege University School of Dentistry Department of Periodontology, Izmir, Turkey, 2 University of Goteborg, Sweden Objectives: Smoking negatively affects periodontal health. Hormonal changes which occur during pregnancy have also been reported to have adverse effects on the periodontal tissues or indirectly through alterations in subgingival bacterial flora. At present, no knowledge exists concerning possible effects of smoking on composition of subgingival plaque in pregnancy. The purpose of the present study was to evaluate effects of smoking during pregnancy on subgingival plaque bacteria most commonly associated with periodontal disease. Methods: A total number of 181 women were examined within 72 hours post-partum. Smoking status was recorded by means of a self-reported questionnaire and the study population was divided into three groups; non-smokers, light-smokers, and heavy-smokers. In each woman two subgingival plaque samples were obtained from mesio- or disto-buccal aspect of randomly selected one molar and one incisor tooth by sterile paperpoints. Clinical periodontal recordings of dental plaque, bleeding on probing (BOP), and probing pocket depth (PPD) were performed at 6 sites per each tooth at all teeth. Plaque samples were analyzed by checkerboard DNA-DNA hybridization with respect to 12 bacterial species. In all analyses, the individual subject was the computational unit. Thus, mean values for all clinical parameters were calculated and bacterial scores from each individual sample were averaged. Statistical methods included Chi-square test, Kruskall-Wallis test and Mann-Whitney U test. Results: Mean ages were similar in the study groups. Plaque, BOP and PPD recordings were lower in the heavy-smoker group, but the differences were not statistically significant (P>0.05). The detection rates and bacterial loads of the specific subgingival bacteria exhibited no significant differences between the groups. No correlation could be found between smoking status and detection rates and bacterial loads of various bacterial species. Conclusion: The present findings suggest that smoking during pregnancy does not have a significant effect on composition of subgingival plaque bacteria. | ||
| Seq #46 - Periodontal Research 11:30 AM-1:30 PM, Saturday, 28 August 2004 Crowne Plaza Hotel SEDEF I | ||
|
Back to the Scientific Program Program
| ||