Objectives: The present study was planned to comparatively analyze total proteoglycan (PG) and chondroitin-4-sulfate (C4S) levels in CsA-induced overgrown gingival tissue samples obtained before and after initial periodontal treatment and to compare these findings with the situation in healthy gingiva. Methods: Gingival tissue samples were obtained from 9 patients with CsA-induced gingival overgrowth before and 4 weeks after initial periodontal treatment including oral hygiene instruction and scaling and also from 10 healthy control subjects. Total PG and C4S levels were determined by biochemical techniques. PG levels were analyzed using modified Bitter and Muir method. C4S assay was carried out using chondroitin sulphate lyase AC and chondroitin-6 sulphate sulphohydrolase enzymes. The results were tested statistically using non-parametric tests. Results: All clinical measurements in the CsA-induced gingival overgrowth group demonstrated significant reductions 4 weeks after initial periodontal treatment (P<0.05). Baseline total PG level in the CsA-overgrown gingiva was higher than the healthy control group, but the difference was not statistically significant (P>0.05). The gingival tissue levels of PG in CsA-induced gingival overgrowth group decreased significantly 4 weeks after treatment (P=0.043). Gingival tissue C4S levels in the overgrowth group were significantly higher than the healthy control group at baseline (P=0.000). C4S levels of the overgrowth group were significantly reduced after treatment (P=0.033), but these levels were still significantly higher than the healthy control group (P=0.000). Conclusion: The observed prominent increase in gingival tissue C4S levels and insignificant increase in PG levels may be interpreted as a sign of an increase in C4S synthesis in CsA-induced gingival overgrowth. Furthermore, remission of clinical inflammation by means of initial periodontal treatment had a positive effect on tissue levels of these extracellular matrix molecules.

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