Aim: The purpose of this study was to investigate the effect of dental fluorosis on micro-shear bond strength of two etch and rinse composite materials to enamel. Materials and Method: Six extracted human third molar teeth with fluorosis and six extracted human third molar teeth with non-fluorosed were used in this study. The teeth were removed from soft tissue remnants and cleaned with fluoride free pumice and rubber cup. The teeth were also divided into two bonding groups (Prime Bond NT, Esthet-X and Pekabond, Pekalite). These materials were applied to enamel incrementally according to manufacturers' instruction. The specimens were exposed to 1000 thermo-cycles between 5 and 55 0C. For the micro-shear bond test were prepared 20 sticks of 1mm2 from each group. The sticks were stressed in shear at a speed of 1 mm/min with a micro-shear test device. After failure, maximum load was recorded and the fracture surfaces were examined under a stereo microscope. Results of the micro-shear test and failure modes were analyzed with Kruskal-Wallis and the Mann-Whitney U test. Results: The mean bond strength of Prime Bond NT was 11.43±5.34 MPa for non-fluorosed teeth, and 9.85±4.54 MPa for teeth with fluorosis. The mean bond strength of Pekabond was 8.51±3.20 MPa for non-fluorosed teeth, and 7.20±2.66 MPa for teeth with fluorosis. Although the adesiv systems showed higher bond strength on normal enamel groups than fluorosis groups, the difference between groups was not statistically significant (P>0.05). Adhesive mode of failure was the most prevalent in all groups (P>0.05). Conclusion: As a result of this study, the etch-rinse type bonding materials used in this study affected negatively by dental fluorosis. However, they presented similar bond strength on both of enamel type.

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