| 0343 Light Polymerization Influence on Living Cells in Pulp Chamber Model | ||
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A. UHL, EMS SA Electro Medical Systems, Nyon, Switzerland, B.W. SIGUSCH, Friedrich-Schiller-Universität Jena, Germany, and A. VÖLPEL, Friedrich Schiller University, Jena, Germany Objectives: The aim of this study was to test the hypothesis that the use of a fast curing halogen light curing unit (FC-LCU) would result in a statistically significant lower number of living human gingiva fibroblasts within a pulp chamber model than the application of an LED LCU. It was also hypothesized that the cytotoxicity of the samples cured with a FC-LCU is statistically significant greater than the samples cured with an LED LCU. Methods: The composites Admira, Grandio, Filtek Supreme and Filtek Z250 were polymerized with the LCUs Swiss Master Light, Optilux 501 and an LED LCU prototype on top of a pulp chamber model. The temperature was recorded within the pulp chamber with a thermocouple. The cytotoxicity of the polymerized samples was tested by using the MTT test. Results: There was no statistically significant difference in the cell number (p=0.3767) if the different LCUs were used. Statistically significantly fewer living cells (p<0.0000) were found if the composite Supreme was used instead of one of the other three composites. The cytotoxicity test of the polymerized samples showed no statistically significant difference in the cell number (p=0.1730) for the different LCUs and in general no statistically significant difference in the cell number for the different composites. Conclusions: Using a FC or a standard halogen or an LED LCU did not result in a considerable difference in the temperature increase or the number of living cells within a pulp chamber model. The composite had surprisingly a statistically significant influence of the number of living cells in the pulp chamber model. Therefore, this study indicates that not only the temperature may have an effect on living cells, but also that cells may be negatively affected by the unpolymerized composite or the light of the LCUs which is absorbed differently by the composites. | ||
| Seq #33 - Dental Materials: Material/ Tooth Interface, Toxicity 4:00 PM-6:00 PM, Friday, 27 August 2004 Crowne Plaza Hotel SEDEF III | ||
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