Objectives: One of the most important goals of the periodontal treatment procedures is to stimulate bone remodelling by resorption and formation of new bone into osseous defects resulted from periodontal disease. A novel approach to achieve this aim is the use of platelet-rich plasma which is known that contains several growth factors at high levels. The purpose of this study was to investigate the response, related to proliferation and collagenase secretion, of osteoblasts derived from a patient with aggressive periodontitis to the homologous PRP, in order to evaluate the role of PRP in periodontal regeneration.

Methods: Cells cultures were derived from alveolar bone chips obtained from a patient with aggressive periodontitis during periodontal surgery and a clinically healthy subject during surgical procedure for crown lengthening. PRP obtained from 1 healthy volunteer was prepared from plasma by centrifugation. Cells were grown in 24-well dishes and on day 2 of quiescence, were treated with 0.1, 1 and 5% of PRP. To determine the effect on cell proliferation, DNA synthesis was estimated by measuring [3H] thymidine incorporation. Collagenase secretion, after a 6-hour treatment with PRP and subsequent 48-hour incubation in serum-free medium, was estimated by gelatin zymography.

Results: The results of this study showed that the addition of 5% of PRP in the affected osteoblastic cells cultures increased significantly (p<0.01) the cell proliferation rate compared to untreated control cells cultures. Furthermore, the addition of PRP seemed to activate significantly the MMP-2 secretion from the affected as well the healthy cells.

Conclusion: PRP promotes the cell proliferation and modulates MMP-2 secretion from the osteoblasts derived from sites affected by aggressive periodontitis. It might be concluded that PRP could serve as a useful adjunct in the periodontal treatment and further studies are required to determine the effective role of PRP on aggressive periodontitis therapeutic approach.

Back to the Scientific Program Program
Back to the Joint Meeting of the Continental European, Israeli, and Scandinavian (NOF) Divisions of the IADR (August 25 -- 28, 2004)

Top Level Search