Objective: Purpose of this 2-way, double-blind, cross-over, in-situ study was to evaluate the remineralization of demineralized enamel specimens in artificial approximal spaces using a 250 ppm fluoride-mouthrinse (Elmex anticaries mouthrinse, 100 ppm F- as amine fluoride, 150 ppm F- as sodium fluoride, Muenchenstein, Switzerland) compared to a placebo-mouthrinse. Methods: 24 volunteers received intraoral approximal appliances with mounted demineralised enamel specimens facing towards natural tooth surfaces, thereby forming an artificial approximal space. Each four specimens were located between the last premolar and the second molar on both sides of the lower jaw. The volunteers rinsed their mouth twice a day (10ml/30s) with the respective mouthrinse. The specimens were cleaned once a day to simulate the use of dental floss. After 28 days mineral gain and fluoride uptake were determined. Statistical analysis was performed by ANOVA. Results: Mineral gain was significantly higher after the use of the fluoride-mouthrinse [569.90 Vol. %•µm] compared to placebo treatment [27.85 Vol. %•µm]. The fluoride treatment [4066.4 µg/cm³] resulted in a significantly higher amount of fluoride uptake compared to the placebo- [596.8 µg/cm³] mouthrinse. Conclusion: The use of the fluoride-mouthrinse supports remineralization of initial caries lesions even in areas difficult to access. This study was supported by GABA international.

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