Objectives: The aim of this study was to determine the effect of three various storage solutions and two storage duration on the micro-shear bond strength (MSBS) of resin composite. Methods: Immediately after extraction, sixty non-carious human permanent molars were divided into three groups for storage solutions (0.1 % thymol, 10% formalin, distilled water). Each tooth was separated mesio-distally to two parts. The first parts were stored for 24 hours, and the second parts were stored for 2 months in the solutions at room temperature. After storage periods, enamel surfaces were covered by a hybrid composite (36% phosphoric acid, Prime&BondNT, TPH) according to the manufacture's instructions. The restored teeth parts were replaced in its respective storage solution for 24 hours. The bonded specimens were serially sectioned into sticks by a water cooled diamond saw. The bond area of the sticks was approximately 1 mm˛. The specimens were subjected to the MSBS test. The data was analyzed by one way ANOVA –Tukey HSD and Paired sample t test. Results: The mean values of tymol, formalin and distilled water groups were 7.35±2.28, 8.48±2.38 and 9.24±2.56 MPa for 24 hours, and 8.08±1.47, 9.25±1.75 and 9.70±2.32 MPa for 2 months, respectively. There was no statistical difference between two storage periods for each storage solutions (p>0.05). The specimens stored in thymol solution showed lower MSBS than those of distilled water for 24 hours and 2 months (p<0.05). The MSBSs of the formalin groups were between thymol and distilled water groups for two storage periods and there were no statistical differences (p>0.05). Conclusion: As a result of this study, the thymol solution caused decrease of MSBS of the resin composite when compared to the formalin and distilled water at 24 hours and 2 months. MSBS of the resin composite was not affected by storage duration.

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