Objective: To compare the antibacterial activities of two dentin bonding systems, Clearfil Protect Bond primer (Kuraray, Japan) and Xeno III (Dentsply DeTrey, Germany) by agar well, dentin and paper discs and a cavity tooth model.

Method: In the well technique, the test materials were filled in the agar wells of plates inoculated with Streptococcus mutans (CCUG 6519). The paper discs were embedded in adhesives and placed on the seeded agar plates. The adhesives were applied on the dentin discs and placed in holes in the plates. After 48 hours, the zones of inhibitions were measured in milimeters. For the cavity tooth model test, three cavities were prepared in the flat occlusal dentin of human extracted molars. The teeth were left in broth culture of S. Mutans at 37 ºC for 72 hours allowing bacteria to invade. The DBS were applied in the same manner as clinical application and light-cured on each of the two infected cavities and the third was left unapplied for control. The teeth were kept in saline for 72 hours. Standart amounts of dentin chips were obtained from the cavity walls and the number of bacteria recovered was counted.

Results: The results were analyzed by factorial ANOVA and Duncan multiple comparison test. For all the methods tested, Clearfil Protect Bond primer exhibited greater inhibition zones than Xeno III (p< 0.05). When tested by the cavity method, the application of Clearfil Protect Bond resulted in significantly less bacterial recovery than Xeno III (p< 0.05).

Conclusion: The cavity tooth model method used in this study was effective for evaluating the antibacterial effects of DBS and the Clearfil Protect Bond System was able to inactivate the bacteria in the cavity effectively in comparison with little antibacterial activity shown by Xeno III. The adhesive systems were generously donated by the manufacturers.

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