Proliferation activity in actinic cheilitis

*0567 Proliferation activity in actinic cheilitis*
A. POULOPOULOS¹, A. EPIVATIANOS¹, A. KIZIRIDOU², and D. ANTONIADES¹, ¹ Aristotle University of Thessaloniki, Greece, ² Theagenion Anticancer Hospital of Thessaloniki, Greece Objectives: Ultraviolet radiation of the vermilion border of the lips can precipitate either varying degrees of dysplasia or undergo malignant transformation into squamous cell carcinoma (SCC). The aim of this study was to elucidate cellular mechanisms involved in malignant transformation of actinic cheilitis. Methods: Expression patterns for markers of cell proliferation Ki-67 antigen, Proliferating Cell Nuclear Antigen (PCNA) were studied in 12 cases of actinic cheilitis, 12 cases of actinic cheilitis with variable degree of epithelial dysplasia, 12 cases SCC of the lips, and 6 cases of normal lip specimens. An immunohistochemical technique (peroxidase-antiperoxidase) was carried out with monoclonal antibodies Ki-67, PCNA (Novocastra laboratories Ltd). Epithelial dysplasia was diagnosed according to the cytologic features listed in the World Health Organization criteria. The distribution of immunostained changes in the epithelium was evaluated, and Ki-67, PCNA positive cells were quantified in selected areas. All data were tabulated and statistical evaluation was performed. Results: The expression of both Ki-67, PCNA was nuclear in all specimens. Although the expression was mostly limited to the basal cells or the immediate suprabasal cell layers in normal epithelium, it was more widely distributed in actinic cheilitis with dysplastic epithelium. Both markers tended to increase with the severity of dysplasia and the progression to SCC. A significant association was observed between the presence and severity of epithelial dysplasia in actinic cheilitis and the suprabasal expression of Ki 67 and PCNA. Conclusion: Our results suggested that overexpression of Ki-67, PCNA positive cells correlated with the degree of epithelial dysplasia in actinic cheilitis lesions. A combined analysis of Ki-67, PCNA expression may provide detailed insight into cell proliferation in actinic cheilitis and the possible malignant transformation into SCC.
Seq #56 - Oral Medicine and Pathology II 4:00 PM-5:30 PM, Saturday, 28 August 2004 Crowne Plaza Hotel AVSA I
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