Objectives: To assess the in vitro biocompatibility of an apatite-mullite glass-ceramic (LG112), using a rat osteosarcoma (ROS) cell line.

Methods: Sample discs of LG112 glass and glass-ceramic based on the formula: 1.5SiO₂ - Al₂O₃ – 0.53P₂O₅ - CaO – 0.67CaF₂ were produced using the lost wax casting technique. Half were studied in the as cast state and others were polished to assess the effect of surface finish on biocompatibility. ROS cells were cultured in the presence of biomaterials for up to four days. Discs of gypsum and phosphate bonded refractory materials were also assessed for biocompatibility in the absence of glass-ceramic. Evaluation of in vitro biocompatibility was carried out using scanning electron microscopy, total protein and MTT assays.

Results: Samples of LG112 glass and glass-ceramic showed good in vitro biocompatibility using both SEM and cell growth and viability assays. Polishing samples appeared to show reduced biocompatibility in comparison to as cast samples. However both refractory materials showed significant cytotoxicity.

Conclusions: Apatite-mullite glass-ceramics showed good in vitro biocompatibility using an osteoblast-like cell culture system. These materials may therefore show potential for use as prostheses for tissue repair or augmentation. In addition, the cytotoxicity of refractory materials highlighted the importance of careful removal of residual material after casting.

Back to the Dental Materials Program
Back to the BSDR Annual Scientific Meeting (6th-8th April 2004)

Top Level Search