Objectives: The aim of this study was to evaluate the apical sealing of materials of mineral trioxide aggregate using the Rhodamine B ink solution. Methods: Forty-three extracted human canines were used. The root canals were instrumented and filling. After the root apical section, retrograde cavities were prepared and the teeth randomly divided into 3 experimental groups (n=13) and two controls (n=2). The following retrofilling materials were used: group 1- Pro-Root MTA (Tulsa/Dentsply); group 2- MTA Angelus of fast cure; group 3- Zin Oxide and Eugenol cement. Then, the teeth were immersed in 0.2% Rhodamine B solution for 48 hours in vacuum environment. After this period, the roots were longitudinally sliced and the leakage was analyzed using perfil projector. Results: The positive and negative control groups presented maximum or no leakage respectively. The results were analyzed with Kruskal-Wallis and Dunn's tests (Ą= .05). The three materials showed similar apical sealing ability (p= 0.05). Conclusion: It was concluded that the materials of mineral trioxide aggregate show similar apical sealing ability to the ZOE using the Rhodamine B ink solution.