| 1191 Adaptive response of hMSC-derived osteoprogenitors to LPS enriched environment | ||
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K.H.K. YIP, I.F. MO, H.K. LAW, Y.L. LAU, and G.C. CHAN, University of Hong Kong, Hong Kong, Hong Kong Objective: To investigate the cellular responsiveness of hMSCs osteoprogenitors to both the gram-positive and gram-negative endotoxins: lipoteichoic acid (LTA, Streptococcus pyogenes) and lipopolysaccharides (LPS, Escherichia coli). Methods: The cellular responsiveness of hMSC-derived osteoprogenitors to LTA and LPS was evaluated by the expression of Toll-Like Receptors (TLRs), specifically TLR2 and TLR4, using quantitative PCR. Results: Prolonged LPS challenge upregulated the osteogenic differentiation of hMSCs. TLR2 and TLR4 gene expressed in hMSCs or its derived osteoprogenitors. Conclusions: Under the influence of LPS, the hMSC-derived osteoprogenitors showed a paradoxically reduced TLR2 and TLR4 gene expression pattern. This adaptive response of hMSC-derived osteoprogenitors in a bacterial endotoxin enriched (LPS) environment suggests a possibly regulatory mechanism in maintaining homeostasis. Significance: Our novel findings support the potential of using hMSCs as a biological graft. | ||
| Seq #170 - Microbiology 10:45 AM-12:00 PM, Saturday, April 5, 2008 Hilton Anatole Hotel Trinity I - Exhibit Hall | ||
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