| Seq #245 | Saturday, March 24, 2007 | |||||||||||||||||||||||
| 9:00 AM-10:30 AM Ernest N. Morial Convention Center 298, Symposium - Group/Division Sponsored | ||||||||||||||||||||||||
| Applications of High-resolution Nuclear Magnetic Resonance (NMR) Analysis in Oral Sciences | ||||||||||||||||||||||||
Sponsored by: Cariology Research, Dental Materials, Geriatric Oral Research, Implantology Research, Microbiology / Immunology and Infection Control, Mineralized Tissue, Nutrition, Oral Health Research, Oral Medicine & Pathology, Periodontal Research, Pharmacology, Therapeutics, & Toxicology, GRINrx Corp | ||||||||||||||||||||||||
| Description: High-resolution, high-field NMR spectroscopy is now an established analytical tool which has proven extremely valuable for the purpose of probing the metabolic status of biofluids & tissues. Indeed, this technique offers many advantages over alternative analytical methods which are often time-consuming, relatively labour-intensive & destructive. These advantages include (1)its ability to provide simultaneous multicomponent information regarding the metabolic status of biofluids, together with those of whole (intact) tissues or appropriate extracts derived therefrom; (2) its virtual non-invasive nature; (3) the involvement of only a minimal level of sample preparation prior to analysis; (4) its high degree of spectral dispersion and sensitivity (the latter now less than or equivalent to ěmol. per litre concentrations); (5) the rapid identification of endogenous or exogenous agents, a process facilitated by the presence of 2 or more “fingerprint” signals (spin-spin coupled if the nuclei, H-1 or otherwise, are adjacent to each other) in NMR spectra acquired on such complex, multicomponent samples; and (6) the rapid detection & determination of agents which would not generally be anticipated to be present in these matrices (e.g., the toxic agents methanol & acetone in human saliva arising from the direct and/or passive inhalation of cigarette smoke). Although the organic acid concentrations of human saliva specimens have been previously monitored by a range of alternative analytical techniques, for example gas-liquid chromatography (GLC), column chromatography, & high performance liquid chromatography (HPLC), such methods require much information regarding the precise molecular nature of such components prior to analysis. Therefore, these techniques only offer an extremely limited characterisation of the metabolic status of this biofluid (and also those of, for example, carious dentin and plaque), & are essentially sample-destructive. The multicomponent analysis of specimens collected from the oral environment by NMR spectroscopy therefore offers many research benefits. | ||||||||||||||||||||||||
| Chairperson: M.C. GROOTVELD | ||||||||||||||||||||||||
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Back to the IADR/AADR/CADR 85th General Session and Exhibition (March 21-24, 2007)