Prevalence of HPV-32 in the Oral Cavity of HIV+ Individuals

*1167 Prevalence of HPV-32 in the Oral Cavity of HIV+ Individuals*
N. HERREL¹, J. CAMERON², N. LINDSEY¹, C. CHEEKS¹, K. SUTTON¹, J. LEIGH¹, P. FIDEL¹, and M. HAGENSEE¹, ¹Louisiana State University, New Orleans, USA, ²Tulane University, New Orleans, LA, USA Introduction: Human Papillomavirus-32 (HPV-32) has been associated with focal-epithelial-hyperplasia (FEH). Previous data from our laboratory has shown that 65% of HIV+ individuals with oral warts contain HPV-32 DNA. The prevalence of HPV-32 in the general public as well as in HIV+ community in those without oral warts has not been well studied. The goal of this study is to define the prevalence rate of oral HPV-32 in a cohort of HIV+ individuals and to better define the site of oral infection. Methods: A total of 179 HIV+ individuals were enrolled from the New Orleans area. Each patient had 7 swabs (cheek, gums, roof, tonsils, tongue, and under tongue) and 2 fluid samples collected (saliva and gargle). An HPV-32 L1 specific polymerase chain reaction (PCR) assay was developed which yielded a 134bp amplicon which was visualized on a 2.5% agarose gel. A questionnaire about basic demographic information, sexual practices, and history of dental hygiene was collected as well as current peripheral blood CD4 cell count and HIV viral load. Results: Overall, a 38% prevalence rate of HPV-32 in HIV+ individuals was seen. There was no predilection for any particular oral cavity site (cheek-12%, gums-12%, roof-11%, tonsils-15%, tongue-12%, under tongue-10%, saliva-13%, and gargle-21%). Risk factors for HPV-32 infection are currently being analyzed. Using a less sensitive hybridization assays to detect 28 HPV-types, HPV-32 prevalence was 9% and the next highest prevalence was HPV-83 at 6%. Conclusions: HPV-32 has a high prevalence rate in the HIV+ community when compared to published studies on a healthy HIV-negative population from South Africa (22.7%). This is consistent with the majority of oral warts being associated with this virus. The propensity for HPV-32 to infect the oral cavity of HIV+ individuals and to cause disease predominantly in them is currently under investigation. NCI 1-R03CA11132-01 NIH 1-P20RR020160-01
Seq #126 - Microbes and Oral Infections 3:30 PM-4:45 PM, Thursday, March 22, 2007 Ernest N. Morial Convention Center Exhibit Hall I2-J
Back to the Microbiology / Immunology and Infection Control Program Back to the IADR/AADR/CADR 85th General Session and Exhibition (March 21-24, 2007)
Top Level Search