| 2281 Effect of Ca(OH)2 and Er:YAG laser on endotoxin | ||
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R.A.B. SILVA, P. NELSON-FILHO, S. ASSED, J.D. PECORA, A.I. MEDEIROS, L.H. FACCIOLI, and R.S. ROCHA, Universidade de São Paulo, Ribeirão Preto, Brazil Objective: The aim of this study was to evaluate, in vitro, the effect of calcium hydroxide and Er:YAG laser on bacterial endotoxin (LPS), by nitric oxide detection in macrophages cell line culture. Background data: Er:YAG laser antimicrobial effect is being investigated and praised in substitution of intracanal dressing or preceding it. However, the laser effect on bacterial endotoxin has yet to be determined. Methods: Samples of LPS solution (50µg/mL), calcium hydroxide suspension (25mg/mL), and LPS suspension with calcium hydroxide were prepared. The study was accomplished according with the following groups: I- LPS; II- LPS with calcium hydroxide; III- LPS with Er:YAG laser (15Hz and 140mJ); IV- LPS with Er:YAG laser, (15Hz and 200mJ); V- LPS with Er:YAG laser (15Hz and 250mJ), VI- Pyrogen-free water; and VII- Calcium hydroxide suspension. A culture of murine macrophages J774 cell line was placed in a 96 wells plate, in witch 10μL of the samples were respectively added. The supernatants were collected for NO detection by GREISS reaction. Results: The averages of NO production were, in μM: I- 10.48±0.58; II- 6.41±0.9 ; III- 10.2±0.6; IV– 8.35±0.4 ; V– 10.4±0,53; VI- 3.75±0.7; VII– 6.44±0.6 . The complete experiment was repeated after one week, and the averages were: I- 21.2±1.5; II- 9.1±0.6; III- 19.5±1.0; IV- 18.5±0.6; V- 21.3±0.9; VI- 2.0±0.2; VII- 6.8±1.7. There was no significant difference (p>0,05) between Group I and the laser Groups (III, IV e V). There was also no significant difference when Groups II, VI e VII were compared between themselves (p>0,05). There was significant difference (p<0,05) between the averages of Groups I and II, with higher values for Group I. Group II presented similar NO production to Group VI (p>0,05). Conclusions: Calcium hydroxide was capable to inactivate bacterial endotoxin, while laser, even with different application parameters, did not present this ability. | ||
| Seq #234 - Clinical Microbiology of the Oral Cavity II 3:30 PM-4:45 PM, Friday, March 23, 2007 Ernest N. Morial Convention Center Exhibit Hall I2-J | ||
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