Susceptibility testing firstly should be done to determine the therapeutic potential of new antimicrobial agents. Objectives: To test reference strains of human oral microorganisms against Plantago major extract. Methods: Thirty strains of bacteria:Actinobacillus actinomycetemcomitans, Actinomyces gerencseriae, israelli, odontolyticus, Campylobacter gracilis, rectus, Capnocytophaga gingivalis, sputigena, Eikenella corrodens, Fusobacterium nucleatum ss nucleatum, nucleatum ss polymorphum, periodonticum, Gemella morbillorum, Neisseria mucosa, Peptostreptococcus micros, Prevotella melanogenica, intermedia, nigrescens, Propionibacterium acnes, Selenomonas noxia, Streptococcus anginosus, gordonii, salivarius, mitis, oralis, sanguis, intermedius and Tannerella forsythensis were tested for their capacity to grow with Plantago major extract. Bacteria were initially grown on agar plates optimized for growth of the specific species. Plates were incubated for 7 days at 35ºC, in 80% N₂, 10% CO₂ and 10% H₂. Aliquots of these samples were used to inoculate eppendorf tubes containing 2% mycoplasma broth (BBL) and placed into 96-well microplates. For the antibacterial assays, medicinal plant extract from South Brazil was dissolved in distiled water to a final concentration ranging (0, 1, 2, 4, 8, 16, 32 and 64 µg/ml). The microplate wells were seeded with 0.1ml of the each bacterial inoculum and the samples were then incubated and growth evaluated by microplate reader at 0, 27, and 68 h under anaerobic conditions by the increase 630 nm absorbance. Results: The results were expressed as the minimum concentration which completely inhibited bacterial growth and exponencial regression curves. Plantago major displayed activity towards the P. melanogenica and Tannerella forsythensis (³64 µg/ml); Peptostreptococcus micros (³16 µg/ml) and Prevotella nigrescens (³1 µg/ml). Selenomas noxia changed your growth curve of y = 0.0533e^0.5907x (R²=0.90) for y = 0.0763e^0.2791x (R²=0.98) when exposed at 1µg/ml of Plantago major. Conclusions: These data indicate that the Plantago major has antibacterial activity against oral bacteria under anaerobic conditions.

Back to the Pharmacology, Therapeutics, & Toxicology Program
Back to the IADR/AADR/CADR 85th General Session and Exhibition (March 21-24, 2007)

Top Level Search