| 1399 3D Imaging of Oral Carcinogenesis with Optical Coherence Tomography | ||
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J. ZHANG, M.J. HAMMER-WILSON, L.-H.L. LIAW, L.H. LI, Y. AHN, Z. CHEN, and P. WILDER-SMITH, University of California - Irvine, USA Objectives: In this study, a three dimensional, high resolution optical coherence tomography (OCT) system and real-time signal processing were evaluated for noninvasive detection and characterization of oral carcinogenesis in the hamster cheek pouch model. Long-term goal is development of a non-invasive system for screening and monitoring patients. Methods: The Golden Syrian hamster cheek pouch model for oral carcinogenesis was used. By application of 0.5% DMBA in mineral oil three times per week, mild to severe dysplasia developed at 3-6 weeks, progressing to squamous cell carcinoma at 10-12 weeks. 3D OCT (with 10 micron resolution) was performed by attaching the anesthetized hamster's everted cheek pouch to a microscope translation stage using a specially designed and fabricated ring-shaped clamp rigidly fastened to the stage surface. This permitted accurate co-localization of imaging and histological data. 3D OCT volume images were reconstructed by scanning an area of 6mm by 6mm based on the 7mm diameter window size in the cheek pouch holder. After optical measurements, the animals were sacrificed to obtain specimens for routine paraffin embedding and H&E, collagen staining. Results: 3D imaging provided better identification of multiple lesions and superior mapping of lesion margins than 2D imaging. In the 3D OCT images, multiple epithelial and subepithelial layers and presence or absence of basement membrane were clearly visible. The 3D OCT images showed overall epithelial thickening, broader rete pegs and loss of polarity in the basal cell layer corresponding closely to H&E stained images. Two-D pullout sections were possible for more detailed viewing in specific areas of interest. Agreement between OCT- and histopathology-based diagnosis using 2 blinded investigators averaged 84-93%. Conclusions: These 3D OCT studies demonstrate the diagnostic potential of this modality in oral tissues. Supported by TRDRP 14IT-0097, CRPF 30003, NIH (LAMMP) RR01192, DOE DE903-91ER 61227, NIH EB-00293 CA91717, NSF BES-86924. | ||
| Seq #167 - Cancer Diagnosis 10:45 AM-12:15 PM, Friday, March 23, 2007 Ernest N. Morial Convention Center 387 | ||
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