| 2722 Confocal Microscopy Evaluation of Standardized Tetracycline-Stained Teeth | ||
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G.S. ROZIER, D.C.N. CHAN, D.W. KIERNAN, W. BROWNING, A.L. STEEN, and S.K. NELSON, Medical College of Georgia, Augusta, USA It has been reported that tetracycline stained teeth will fluoresce bright yellow under UV light. Objectives: The purpose of this study was to evaluate fluorescence of standardized tetracycline-stained teeth bleaching with a 20% hydrogen peroxide system using confocal microscopy Methods: Extracted molars were subjected to discoloration by three tetracycline (TCN) analogs: chlortetracycline, doxycycline and minocycline (Quintessence International, 37 (8) 637-646). Following discoloration, three randomly selected samples from each group were subjected to Zoom2 in-office tooth whitening procedures. The color change of both the buccal (B) and lingual (L) surfaces was monitored weekly for 7 weeks. Two randomly selected samples from each of the untreated and treated groups were selected for high resolution confocal microscopic images (LSM 510 Meta, Carl Zeiss, Oberkochen, Germany) and an argon-488 laser. Samples from each of the four groups were encased in resin and sectioned in half from buccal to lingual. The sectioned samples were placed on a drop of immersion oil on a glass microscope cover slip. High resolution confocal microscopic images were then obtained. Samples were viewed with a 5x/0.16 objective, focusing approximately 150 microns below the sectioned surface. Images were relayed to a computer monitor for viewing. Additional images were made (10x/0.3 objective) at the junction of the enamel and dentin, within the dentin alone, and at the junction of the dentin and pulp chamber. Results: Examples from sectioned chlortetracycline-stained sample (A) naturally occurring chlortetracycline stains (B) unbleached chlortetracycline-stained sample (C) bleached chlortetracycline-stained sample
Conclusions: Standardized tetracycline-stained teeth also fluoresce under confocal microscopy. The pattern of fluorescence is different from naturally stained teeth. Minocycline proved to be a less effective fluorescent marker than Doxycycline and Chlortetracycline. At the prescribed laser wavelength, the samples subjected to minocycline appeared to fluoresce no more intensely than the control teeth. | ||
| Seq #282 - Tooth, Biocompatibility 10:45 AM-12:00 PM, Saturday, March 24, 2007 Ernest N. Morial Convention Center Exhibit Hall I2-J | ||
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