| 1876 Ability of Therapeutic Ozonated Water to Oxidatively Consume Biomolecules | ||
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J.E. SHENBERG, JAMES E SHENBERG DDS INC, Santa Monica, CA, USA, J.C. BLACKBURN, London South Bank Univeristy, England, Uk, C.J. SILWOOD, London South Bank University, United Kingdom, and M.C. GROOTVELD, Queen's University of Belfast, Northern Ireland, Uk Objectives: The powerful microbiocidal activity of ozone (O3) is well known and previous investigations have demonstrated the potential employment of this reactive oxygen species (ROS) as a therapeutic agent in the treatment of dental caries. This ROS is extremely reactive towards selected carious dentine biomolecules, and such reactions are likely to be of relevance to its microbiocidal activity. Therefore, in this study we have employed high resolution proton (1H) nuclear magnetic resonance (NMR) spectroscopy to determine the nature and extent of the oxidation of biomolecules present in carious dentine, plaque and saliva. Methods: Aqueous solutions containing sodium pyruvate, alpha-D-glucose, L-cysteine and L-methionine (4.00 mM) were prepared in 40.0 mM phosphate buffer (pH 7.00) which was rigorously deoxygenated with argon gas prior to use. 5.00 ml aliquots of these solutions were treated with 20.00 ml of ozonated (2 ppm) water [1] These experiments were conducted in triplicate. Matching de-oxygenated solutions of these biomolecules untreated with O3 served as controls. Results: Attack of O3 on a-D-glucose gave rise to formate as a product, and treatment of pyruvate with this oxidant produced acetate and CO2 via an oxidative decarboxylation process. Moreover, the amino acid volatile sulphur compound (VSC) precursors cysteine and methionine were oxidatively transformed to their corresponding primary oxidation products cystine and methionine sulphoxide respectively. Conclusions: Multicomponent analysis of appropriate chemical model systems provides valuable molecular information regarding the reactivity of ozonated water towards oral environment biomolecules. For example, oxidation of cysteine and methionine provides evidence for the ability of ozonated water to combat oral malodour since these biomolecules are precursors to volatile sulphur compounds (VSCs). [1] TherOzone Unit, U.S.A. | ||
| Seq #205 - PTT Poster Session #2 2:00 PM-3:15 PM, Friday, March 23, 2007 Ernest N. Morial Convention Center Exhibit Hall I2-J | ||
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