| 1875 Therapeutic Ozonated Water Reduction of Microbial-Derived Components in Dental Units | ||
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K.L. JULIAN1, J.C. BLACKBURN2, C.J. SILWOOD1, and M.C. GROOTVELD3, 1London South Bank University, United Kingdom, 2London South Bank Univeristy, England, Uk, 3Queen's University of Belfast, Northern Ireland, Uk Objectives: Dental unit water lines (DUWLs) are frequently contaminated with micro-organisms, and the level of such biofouling often exceeds limits specified by the EU for drinking water, and also by those set by the US FDA for DUWL quality. In this investigation, a multicomponent evaluation of biomolecules present in DUWL specimens was conducted by high resolution proton nuclear magnetic resonance (NMR) spectroscopy in order to examine the ability of this technique to provide useful molecular information regarding the nature and level of microbial contamination therein and to assess the effect of the addition of ozonated water. Methods: DUWL samples were collected from 8 independent dental units, thoroughly centrifuged, and the supernatants subjected to proton NMR analysis using a Bruker AMX-600 spectrometer. One ml of each DUWL sample was added to 10 ml of ozonated water from the commercially available TherOzone unit (USA), and the above process was then repeated. Results: Results acquired revealed that many biomolecules were detectable in the samples examined, including a wide range of microbial fermentation products (MFPs). For example, the organic acid anions acetate, formate, lactate, propionate and succinate, and occasionally ethanol were present in the samples examined. Further components detectable included the amino acids alanine and glycine, and also a series of aromatic compounds. Treatment of DUWLs with the powerful microbicidal agent ozonated water gave rise to a substantial reduction in many of the MFPs detectable in samples analysed 72 hr. after treatment. Conclusions: High field NMR spectroscopy provides much valuable information regarding the chemical composition of DUWLs, data which is likely to reflect the nature and level of their microbial contamination, and the ability of microbicidal agents to diminish such biofouling. Moreover, ozonated water reduces microbial fermentation products in dental unit water lines. | ||
| Seq #205 - PTT Poster Session #2 2:00 PM-3:15 PM, Friday, March 23, 2007 Ernest N. Morial Convention Center Exhibit Hall I2-J | ||
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