| 1170 Roles of produced hTNF-a in HIV-1 inhibition by Lactoferrin | ||
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A. TOMINAGA1, Y. KOMORI1, J. NAKAJIMA1, H. CHIBA1, and H. SENPUKU2, 1Tokyo Medical University, Japan, 2National Institute of Infectious Diseases, Tokyo, Japan Objectives:Lactoferrin plays as an inhibitory agent in salivary components to HIV-1 infection as well as Lactoperoxidase. We investigated relationship between inhibiting effect by Lactoferrin to HIV-1 infection and production of human tumor necrosis factor (hTNF-a) from phytohemagglutinin(PHA)-blasted peripheral blood mononuclear cells(PBMC) in the culture with or without HIV-1.Methods:PHA-blasted PBMC was cultivated with and without TCID 10, 50 and 250 HIV-1MN /ml, which was treated with 0, 25, 50, 100 or 300 mg/ml Lactoferrin, at 1 x 106 cells/ml in 10%FCS RPMI 1640 containing 1 unit IL-2/ml (IL-2-medium) in 48-well plates under a humidified atmosphere of 5% CO2 for 1 day at 37oC. After washing with HBSS 3 times, the infective PBMC were still cultivated in 1 ml of fresh IL-2-medium. The culture supernatants were obtained as samples after 7 days culture. The infection rates of HIV-1 were analyzed by p24 antigen measurement using chemiluminescent enzyme immunoassay,and productions of TNF-a were evaluated utilizing ELISA, in these samples. Expression of TNF-a mRNA in PBMC was also analyzed by RT-PCR.Results:More than 30% inhibitions to P24 production and productions of more than 600 pg/ml TNF-a were observed in the culture of blasted PBMC and HIV-1 treated with 100 or 300 mg/ml of Lactoferrin, in comparison with non-treated control. Moreover, in the culture condition without HIV-1, the production of TNF-a was higher in the sample applied with 100 or 300 mg/ml Lactoferrin (780.1 ± 106.1 pg/ml, p=0.124 or 902.1 ± 29.0 pg/ml, p=0.001) than that (662.4 ± 63.4) in non-applied control. The expressions of TNF-a mRNA in PBMC samples were also confirmed.Conclusion:The inhibition to HIV-1 infection was induced in dose-dependent manner of TNF-a production from PBMC stimulated by Lactoferrin. Lactoferrin possessed both the distinctive activities to inhibition of HIV-1 infection and induction of TNF-a. | ||
| Seq #126 - Microbes and Oral Infections 3:30 PM-4:45 PM, Thursday, March 22, 2007 Ernest N. Morial Convention Center Exhibit Hall I2-J | ||
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