Plaque fluoride concentrations ([F]) are directly related to plaque calcium concentrations (Caries Res 36:256-265,2002). Our attempt to increase plaque F uptake from dentifrices using a 20mM CaCl₂ pre-rinse was not successful (Caries Res 39:100-107,2005). However, a 150mM Ca-lactate pre-rinse significantly increased salivary [F] at 1.0 h after a NaF rinse (Vogel et al, Caries Res 40:449-454,2006). Objective: To determine the effect of a 150mM Ca-lactate pre-rinse on both salivary and plaque [F] after use of a 228-ppm F rinse. Methods: The subjects (n=10) refrained from toothbrushing overnight before each of the two study days. On the first day they rinsed for 1.0 min with 20mL of Ca-lactate and then for 1.0 min with 20mL of the NaF solution. Plaque and whole saliva samples were collected 15 and 60 min later. The procedure was repeated one week later but without the Ca-lactate pre-rinse. Saliva was analyzed with the electrode after HMDS-facilitated diffusion. Plaque F was extracted with five 30-min acetate buffer extractions (pH=4.8) and then by HMDS-facilitated diffusion. Statistical analysis was done using RM-ANOVA. Results: The 15 and 60-min salivary [F] (mean±SE) after use of the NaF rinse were 0.22±0.09 and 0.027±0.007 mM; after the Ca-lactate/NaF rinses they were 2.69±0.76 and 1.06±0.21 mM (p<0.001). The amounts of F extracted from the 15 and 60-min plaque samples with acetate buffer or HMDS diffusion did not differ significantly. The averages of the combined 15 and 60-min amounts extracted with acetate buffer were 9.61±0.97^a (NaF) and 45.0±5.4^b (Ca-lactate/NaF) nmol. The amounts extracted with HMDS diffusion were 31.1±7.3^a and 131.4±20.5^b nmol, respectively. The total amounts extracted were 40.7±7.4^a and 176.4±24.2^b nmol, respectively. Conclusion: Unlike our findings with dentifrice, the use of the Ca-lactate rinse before the NaF rinse significantly increased salivary and plaque [F].  Supported, in part, by NIH/NIDCR Grant #GORMB0020A.

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