Nystatin is an antifungal drug commonly use for the treatment of denture stomatitis, however, its preparation in the form of vaginal tablet has not been evaluated. Objectives: to evaluate the antifungal activity against Candida albicans of the nystatin vaginal tablet combined with a tissue conditioner during a 7-day period. The effect of incorporations of nystatin on the physical property of the tissue conditioner was also examined according to the ISO penetration test. Methods: The specimen disks (13x2 mm) of combined GC Softliner and nystatin were prepared using the concentrations of 3 times (o.6 grams) and 4 times (o.8 grams) of the minimum inhibitory concentration (MIC) value. Non-nystatin group was used as control. Specimens were contaminated with C. albicans (105 cell/ml, 50 µl) for 2 hours, then rinsed and cultured for evaluation (colonie count) of the remaining viable C. albicans. The antifungal effect was evaluated at day 0, 3 and 7, respectively. Results: The MIC of nystatin combined GC Softliner was 3,570 unit/gram. For 0.6 gram-nystatin group after specimen preparation at day 0, 3 and 7, the degree of reduction of CFU versus control were 99.9%, 99.7% and 99.6%, respectively; and the rate of negative culture were 91.7%, 66.1% and 47.2 %, respectively. For 0.8 gram-nystatin group after specimen preparation at day 0, 3 and 7, the degree of reduction of CFU versus control were similar, but the rate of negative culture were increased to 95%, 75% and 66.1 %, respectively. However, the incorporation of 0.8 gram of nystatin altered the physical property of tissue conditioner. Conclusions: It can be concluded that the combination of nystatin vaginal tablet and GC Softliner could effectively reduce high amount of Candida albicans during a 7-day period. These results suggest the possibility for the use of nystatin vaginal tablet for the treatment of denture stomatitis.

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