Objectives: Clinical observations found that patients suffered from bacterial-induced inflammation which result in serious periodontal disease have gradually increased over the years. Lipopolysaccharide (LPS), found in the cell wall of bacteria, was the toxic substance responsible for such periodaontal disease. However, drugs developed based on the above mechanism showed no significant clinical breakthrough. This study aims to investigate the probability of increasing the survival rate of static magnetic field (SMF)-exposed mice challenged with LPS.

Methods: In this study, LPS was intravenous injected into BALB/cByJNarl male mice. The endotoxin-treated mice were divided into prevention (anticipatory SMF exposed) and treatment (SMF exposed after challenge of LPS) groups. Static magnetic field with a flux intensity of 4000 G was used as an endotoxicity inhibitor. Mice without SMF exposure were set as control group. The survival rates in each group of the samples were calculated for evaluating the endotoxin tolerance capability of SMF.

Results: Our results indicated that the survival rate of the tested mice was significantly increased due to SMF exposure in advance for 2 hours before LPS challenge. When the mice were challenged with LPS, the 2 days survival rate is 17%, which is significantly lower than the mice with 2 hours SMF pre-exposure (43%, p<0.05). At 48 hours post LPS injection, all the survival mice recovered their health no matter the mice accepted SMF exposure or not.

Conclusion: Based on these results, we concluded that further advanced researches on static magnetic fields in inhibiting LPS-induced excessive immune reaction is valuable.

Back to the Microbiology / Immunology and Infection Control Program
Back to the IADR/AADR/CADR 85th General Session and Exhibition (March 21-24, 2007)

Top Level Search