| 1215 Healing and cytotoxic effects of Psidium guajava (Myrtaceae) leaf extracts | ||
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K.P.S. FERNANDES1, S.K. BUSSADORI1, M.M. MARQUES2, E. BACH1, N. WADT1, E.M. SANTOS1, V.C. PAVESI1, and M.D. MARTINS1, 1Centro Universitário Nove de Julho, São Paulo, Brazil, 2University of Sao Paulo, Brazil Objective: evaluate the wound healing potential (tissue reaction in rats tongue ulcers) and cytotoxic effects (in vitro) of aqueous and hydro-alcoholic leaf extracts of Psidium guajava and of commonly used corticosteroids (Omcilon A TM and Psorex TM). Methods: The healing effect(in vivo) was studied through the clinical and histological evaluation of rat oral mucosa traumatic lesions treated with these substances. The treatment was continued and the rats were sacrificed in 1, 3, 5, 7 and 14 days later; then, the tissue sections stained with haematoxylin eosin were analyzed. The histological evaluation was submitted to a score according to the healing degree with range from 1 (total repair of the epithelium and connective tissue) to 5 (epithelium ulcer and acute inflammatory infiltrate). Kruskal Wallis test was applied for histological score statistical analysis. P-values ≤0.05 were considered statistically significant. For the in vitro study, each substance was applied to mucosa fibroblast cells (FMM1) cultures in conditioned media. The media were conditioned by placing the substances in contact with fresh culture medium for 24 h. The cytotoxicity analysis was performed using the MTT assay. Data were treated by ANOVA (P≤0,05) and Tukey test (P≤ 0,05). Results: In vitro, guava extracts caused a decrease in the cell viability and growth (30% in average) when compared to control and corticosteroids.In vivo, the guava extracts revealed an acceleration on wound healing since third day and corticosteroids showed a delay on tissue repair associated to bacterial surface colonization, presence of micro-abscesses and intense inflammatory infiltrate in the submucosa. Conclusion: The guava extracts in short time cytotoxicity analysis reduced the cell population however,in vivo showed to be biocompatible and accelerated the wound healing. The corticosteroids stimulated the cells proliferation in vitro however in vivo, caused delay on healing and biased toward bacterial tissue infection. | ||
| Seq #129 - Clinical Diseases and Pathogenesis 3:30 PM-4:45 PM, Thursday, March 22, 2007 Ernest N. Morial Convention Center Exhibit Hall I2-J | ||
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