| 1214 Cytotoxicity analysis and healing properties of Chamomilla extract and corticosteroids | ||
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M.D. MARTINS1, M.A.T. MARTINS1, S.K. BUSSADORI1, M.M. MARQUES2, V.C. PAVESI1, M.P.P. FERREIRA1, and K.P.S. FERNANDES1, 1Centro Universitário Nove de Julho, São Paulo, Brazil, 2University of Sao Paulo, Brazil Objective: To analyze the immediate cytotoxicity (in vitro) and the wound-healing properties (in vivo) of this phytotherapic chamomile substance, compared with those of commonly used corticosteroids in topical ulcer treatment. Methods: For the in vitro study, the substances were applied to oral mucosa fibroblast cells (FMM1) that were cultured in conditioned media with Chamomile recutita (L.) Rauschert fluid extract (Ad-Muc®), Omcilon A®, and Psorex® corticosteroids. The data were compared by ANOVA (P ≤ 0.05), and with Tukey's post hoc test (P ≤ 0.05). The healing effect (in vivo) was studied through the clinical and histological evaluation of rat oral mucosa traumatic lesions treated with these substances. The treatment was continued, and the rats were killed 1, 3, 5, 7, and 14 days later. The tissue sections stained with hematoxylin–eosin were then analyzed. The histological evaluation was scored according to the healing degree, with a range from 1 (total repair of the epithelium and connective tissue) to 5 (epithelium ulcer and acute inflammatory infiltrate). The Kruskal–Wallis test was applied for histological score statistical analysis. P-values ≤ 0.05 were considered statistically significant. Results: In vitro, chamomile caused a 36% decrease in cell viability, and cell growth was impaired in cultures of corticosteroids when compared with control. In vivo, the chamomile revealed an acceleration of wound-healing from the third day, and corticosteroids showed delayed tissue repair associated with bacterial surface colonization, the presence of micro-abscesses, and intense inflammatory infiltrate in the submucosa. Conclusion: The chamomile substance, after brief cytotoxocity analysis, was shown to reduce the cell population; however, in vivo it was shown to be biocompatible and accelerated wound-healing. The corticosteroids stimulated cell proliferation in vitro; however, in vivo, they delayed healing and were biased toward bacterial tissue infection. | ||
| Seq #129 - Clinical Diseases and Pathogenesis 3:30 PM-4:45 PM, Thursday, March 22, 2007 Ernest N. Morial Convention Center Exhibit Hall I2-J | ||
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