| 1157 Application of Gatifloxacin-containing Bioresorbable Polymer Ceramic Composite to Osteomyelitis | ||
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T. MIYAI1, A. ITO2, Y. SOGO2, G. TAMAZAWA1, T. MATSUNO1, and T. SATOH1, 1The Nippon Dental University, Tokyo, Japan, 2National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan Local delivery of antibiotics using a bioresorbable polymer has been shown to be effective in the treatment of chronic osteomyelitis. However, the antibiotic resorbable polymer does not necessarry support bone tissue reconstruction. Objective: The purpose of this study is to develop composite materials of porous ceramics of β-tricalcium phosphate (TCP) and poly-ε-caprolactone (PCL) loaded with gatifloxacin (GFLX), which can support bone tissue reconstruction after recovery and be replaced finally by new bone tissue. Methods: Molten PCL was mixed with 1% GFLX at 120°C, and solidified in a disk shape 6.35 mm in diameter and 1.2 mm in thickness. The disk was immersed in 10 mL of Hanks' balanced solution, and incubated at 37°C for 8 weeks. Released GFLX was measured using a UV-visible spectrophotometer. Bactericidal properties of the disks were evaluated by the Kirby-Bauer method using Streptococcus milleri (NCTC7331) and Bacteriodes fragilis (NCTC9343). The molten PCL containing 1% GFLX was loaded in the pores and on the surface of the porous β-TCP ceramic body at 120°C. The PCL and β-TCP composites containing GFLX was implanted in S. milleri and B. fragilis osteomyelitis site induced bilaterally in the mandible of a male New Zealand white rabbit using the Satoh-Heimdahl method. Results: The polymer disks containing GFLX released GFLX for 8 weeks in Hanks' balanced solution, and demonstrated a sufficient in vitro bactericidal activity against S. milleri and B. fragilis. After four and 12 week implantation, no inflammatory cells were observed histologically at the implanted site. Osteoconduction was observed on the surface of the composites. Conclusion: The composites of PCL and β-TCP porous body containing GFLX were shown to be effective to treat S. milleri and B. fragilis osteomyelitis in the rabbit mandible, and to serve as a scaffold for bone tissue reconstruction. | ||
| Seq #126 - Microbes and Oral Infections 3:30 PM-4:45 PM, Thursday, March 22, 2007 Ernest N. Morial Convention Center Exhibit Hall I2-J | ||
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