| 1210 Antibacterial Activity of Some Natural Product against Oral Pathogens | ||
|
Y.-H. YUN1, S.-I. JEONG2, and K.-J. KIM1, 1Wonkwang University, iksan, South Korea, 2Jeonju Biomaterials Institute, South Korea Objectives: To investigate the preventive agents for oral disease with natural product, S.miltiorrhizae, G. biloba, S. flavescens and A. cordata. Methods: Antibacterial active compound was successfully isolated from the chloroform fraction of methanol extract of natural product by repeated column and prep-HPLC on the basis of bioassay-guided fractionation, and their structures were elucidated as tanshinone IIA (from S.miltiorrhizae), anacardic acid, bilobol (from G. biloba), kuraridin and kuraridinol (from S. flavescens) and continentalic acid (from A. cordata) by instrumental analysis using 1D-NMR, 2D-NMR and ESI/LC/MS. We investigated the antibacterial activity against S. mutans, A. actinomycetemcomitans and P. gingivalis by MIC/MBC. Results: The minimum inhibitory concentration (MIC) and minimum bactericidal (MBC) concentration in isolated compounds from natural products were 2.0-16.0 and 4-32 ug/mL respectively. Particularly, MIC/MBC of anacardic acid against S. mutans causing dental caries was determined to be 2.0/4.0 ug/mL. The bactericidal test showed that anacardic acid completely inactivated S. mutans at the concentration 8ug/mL in 1 min. Anacardic acid also significantly inhibited the growth of other cariogenic bacteria such as Streptococcus sobrinus, Streptococcus sanguisand, Porpyromonas gingivalis causing periodontitis. Conclusion: The specific activity and fast-effectiveness of natural products against oral bacteria strongly suggests that it could be employed as a natural antibacterial agent in functional foods or oral care products | ||
| Seq #129 - Clinical Diseases and Pathogenesis 3:30 PM-4:45 PM, Thursday, March 22, 2007 Ernest N. Morial Convention Center Exhibit Hall I2-J | ||
|
Back to the Oral Medicine & Pathology Program
| ||