Objective: To characterize proteins produced by P. gingivalis that lead to selective activation of cellular and/or humoral immune responses involved with inflammatory bone resorption.

Methods: Two antigens, PG_1841 and PG_1236, discovered through T cell expression cloning methodology were tested for their relevance in host immunological mechanisms of defense through recognition by Ag-specific mononuclear cells (proliferation assay and cytokine production) and serum Ab of mice infected with P. gingivalis, and by PBMC of individuals with clinically diagnosed periodontal disease. Lastly, we determined the extent that these recombinant antigens modulate bone resorption through in vivo testing in mice.

Results: Full length, PG_1236 and the truncated form of PG_1841 were produced as recombinant proteins in E. coli host cells and shown to be readily recognized by Ag-specific lymphoid cells from mice infected with P. gingivalis, and by PBMC of individuals with clinically diagnosed periodontal disease, as determined by T-cell proliferation, Interferon ã (IFN-ã) production and Ab production.

Conclusion: Oral pathogenic bacteria, Porphyromonas gingivalis produces proteins that selectively stimulate Th1 responses, which potentially contribute to the pathogenesis of alveolar bone resorption. This work supported by grant DE-13747 from the NIDCR.

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