| 1297 Biological evaluation of sputtered nanoscale titania coatings | ||
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Y. YANG1, Y. LIU1, S.-W. PARK2, H.-S. YANG2, S. AHN2, K.-M. LEE2, N. OH3, J.D. BUMGARDNER4, W.O. HAGGARD4, and J. ONG5, 1University of Tennessee Health Science Center, Memphis, USA, 2Chonnam National University, Gwangju, South Korea, 3University of Tennessee, Memphis, USA, 4University of Memphis, TN, USA, 5University of Texas San Antonio, USA Objective: The project was to evaluate the effect of sputtered titania-coated implant devices on biological responses in vitro. Methods: Ti disks were coated with titania using rf magnetron sputtering. The groups evaluated in this study were as-sputtered titania coatings, sputtered and 600C heat-treated titania coatings, and machined Ti surfaces as controls. Implant surfaces were characterized using XRD, SEM, contact angle and surface roughness measurement. Human embryonic mesenchymal cell (HEPM) was used in this study. Cell adhesion was measured by coulter counter within 4 hours. Cell proliferation and differentiation, as indicated by dsDNA and alkaline phosphatase activity, were measured during 12 days of cell culture. Results: The as-sputtered titania coatings and the heat-treated sputtered coatings exhibited dense surface consisting of nanoscale grains. XRD patterns indicated the presence of Ti, and rutile titania on the coated surfaces, whereas trace amounts of anatase titania were also observed on the heat-treated coatings. Cell adhesion was observed to improve on sputtered nanoscale titania coatings compared to machined Ti surfaces within the first hour. No significant differences in dsDNA and alkaline phosphatase activity were observed between the dense nanoscale coatings and Ti controls. Conclusions: Sputtered nanoscale coatings significantly enhanced cell adhesion within the first hour compared to machined Ti surfaces. In addition, no significant differences in cell proliferation and differentiation were observed between the sputtered nanoscale coatings and controls. | ||
| Seq #147 - Implant Surfaces and Coatings In Vitro 9:00 AM-10:30 AM, Friday, March 23, 2007 Ernest N. Morial Convention Center 387 | ||
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